{"title":"Enzymsubstrate \\ FRETS Peptidbibliotheken","description":"\u003cp\u003eJedes Substrat (Code 3701-v – Code 3719-v) der FRETS-25Xaa-Serie enthält eine hochfluoreszierende 2-(N-Methylamino)benzoyl (Nma)-Gruppe, die an die Seitenkette des Aminoterminalen D-2,3-Diaminopropionsäure (D-A2pr)-Restes gebunden ist und durch eine 2,4-Dinitrophenyl (Dnp)-Gruppe, die an die Funktion von Lysin gebunden ist, effizient gelöscht wird. Xaa steht für eine feste Position jeder der 19 natürlichen Aminosäuren, ausgenommen Cys (\u003ci\u003eim Produktnamen Code 3701-v – Code 3719-v angegeben\u003c\/i\u003e). Eine Mischung aus 5 Aminosäureresten (P, Y, K, I und D) befindet sich an der Yaa-Position zusammen mit einer Mischung aus 5 Aminosäureresten (F, A, V, E und R) an der Zaa-Position für jedes feste Xaa. Dies ergibt eine Peptidmischung von 25 Kombinationen jeder Xaa-Serie, was zu einer kombinatorischen Bibliothek von insgesamt 475 Peptidsubstraten führt. Sowohl Nma- als auch Dnp-Gruppen sind an die Seitenkette der einzelnen Reste gebunden, was die Bestimmung der Spaltstelle durch ein spezifisches Enzym mittels massenspektrometrischer Analyse und Edman-Abbau ermöglicht. Die enzymatische Reaktion sollte durch die Verwendung eines geeigneten\u003cspan\u003e\u0026nbsp;\u003c\/span\u003eInhibitors\u003cspan\u003e\u0026nbsp;\u003c\/span\u003e(\u003cspan\u003e\u0026nbsp;\u003c\/span\u003eLeupeptin\u003cspan\u003e\u0026nbsp;\u003c\/span\u003e,\u003cspan\u003e\u0026nbsp;\u003c\/span\u003e\u003ca title=\"E-64, code 4096\" href=\"https:\/\/peptanova.de\/product\/E-64\"\u003eE-64\u003c\/a\u003e\u003cspan\u003e\u0026nbsp;\u003c\/span\u003eoder\u003cspan\u003e\u0026nbsp;\u003c\/span\u003ePepstatin A\u003cspan\u003e\u0026nbsp;\u003c\/span\u003e) beendet werden. Wir haben bestätigt, dass die FRETS-25Xaa-Serie effektiv für den Assay zahlreicher Proteasen wie Trypsin, Chymotrypsin, Elastase, Thrombin, Papain, Calpain, Pepsin und Thermolysin verwendet werden kann. Sie funktionierten jedoch nicht gut für den Assay von Caspase-3, wahrscheinlich weil sie in jedem Substrat nur drei veränderbare Stellen (Zaa-Yaa-Xaa) haben (Defizit der P4-Stelle). Diese Tatsache deutet darauf hin, dass FRETS-25Xaa möglicherweise nicht für den Assay eines Enzyms mit einem breiten Spektrum an Wechselwirkungsstellen mit dem Substrat anwendbar ist.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/peptanova.de\/wp-content\/uploads\/2022\/10\/frets25xaa-manual.pdf\"\u003e\u003cstrong\u003eDownload: PDF-Datenblatt\u0026nbsp;\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","products":[{"product_id":"frets-25ala","title":"FRETS-25Ala","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785956651350,"sku":"3701-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3701-v.jpg?v=1777022378"},{"product_id":"frets-25arg","title":"FRETS-25Arg","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785956716886,"sku":"3702-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3702-v.jpg?v=1777022378"},{"product_id":"frets-25asn","title":"FRETS-25Asn","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785956749654,"sku":"3703-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3703-v.jpg?v=1777022377"},{"product_id":"frets-25asp","title":"FRETS-25Asp","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785956815190,"sku":"3704-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3704-v.jpg?v=1777022377"},{"product_id":"frets-25gln","title":"FRETS-25Gln","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785956847958,"sku":"3705-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3705-v.jpg?v=1777022378"},{"product_id":"frets-25glu","title":"FRETS-25Glu","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785956880726,"sku":"3706-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3706-v.jpg?v=1777022378"},{"product_id":"frets-25gly","title":"FRETS-25Gly","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785956946262,"sku":"3707-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3707-v.jpg?v=1777022377"},{"product_id":"frets-25his","title":"FRETS-25His","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957011798,"sku":"3708-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3708-v.jpg?v=1777022377"},{"product_id":"frets-25ile","title":"FRETS-25Ile","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957044566,"sku":"3709-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3709-v.jpg?v=1777022378"},{"product_id":"frets-25leu","title":"FRETS-25Leu","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957077334,"sku":"3710-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3710-v.jpg?v=1777022378"},{"product_id":"frets-25lys","title":"FRETS-25Lys","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957110102,"sku":"3711-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3711-v.jpg?v=1777022378"},{"product_id":"frets-25met","title":"FRETS-25Met","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957142870,"sku":"3712-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3712-v.jpg?v=1777022378"},{"product_id":"frets-25phe","title":"FRETS-25Phe","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957175638,"sku":"3713-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3713-v.jpg?v=1777022377"},{"product_id":"frets-25pro","title":"FRETS-25Pro","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957241174,"sku":"3714-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3714-v.jpg?v=1777022378"},{"product_id":"frets-25ser","title":"FRETS-25Ser","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957306710,"sku":"3715-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3715-v.jpg?v=1777022377"},{"product_id":"frets-25thr","title":"FRETS-25Thr","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957405014,"sku":"3716-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3716-v.jpg?v=1777022377"},{"product_id":"frets-25trp","title":"FRETS-25Trp","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957732694,"sku":"3717-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3717-v.jpg?v=1777022378"},{"product_id":"frets-25tyr","title":"FRETS-25Tyr","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957798230,"sku":"3718-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3718-v.jpg?v=1777022378"},{"product_id":"frets-25val","title":"FRETS-25Val","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957863766,"sku":"3719-V","price":100.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3719-v.jpg?v=1777022377"},{"product_id":"frets-25-std1","title":"FRETS-25-STD1","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957896534,"sku":"3720-V","price":36.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3720-v.jpg?v=1777022377"},{"product_id":"frets-25-std2","title":"FRETS-25-STD2","description":"","brand":"peptanova","offers":[{"title":"1 μmol","offer_id":52785957929302,"sku":"3721-V","price":36.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1008\/7940\/1302\/files\/3721-v.jpg?v=1777022378"}],"url":"https:\/\/peptanova.com\/collections\/frets-peptide-libraries.oembed","provider":"PeptaNova","version":"1.0","type":"link"}