Muscarinic Toxin 3

M.W. 7379.4

C₃₁₉H₄₈₉N₈₉O₉₇S₈

Leu – Thr – Cys – Val – Thr – Lys – Asn – Thr – Ile – Phe – Gly – Ile – Thr – Thr – Glu – Asn – Cys – Pro – Ala – Gly – Gln – Asn – Leu – Cys – Phe – Lys – Arg – Trp – His – Tyr – Val – Ile – Pro – Arg – Tyr – Thr – Glu – Ile – Thr – Arg – Gly – Cys – Ala – Ala – Thr – Cys – Pro – Ile – Pro – Glu – Asn – Tyr – Asp – Ser – Ile – His – Cys – Cys – Lys – Thr – Asp – Lys – Cys – Asn – Glu

Specifc Ligand for Muscarinic Acetylcholine Receptor-4 (M4) Muscarinic toxin 3 (MT3) was originally isolated from the green mamba (Dendroaspis augusticeps) venom and is composed of 65 amino acid residues. Muscarinic toxin-3 shows a high selectivity for the M4 receptor with low affinity to M1 receptor [FEBS Lett., 352, 91 (1994)], and no binding to M2, M3, and M5 receptors [FEBS Lett., 352, 91 (1994), Toxicon, 34, 1257 (1996), Life Sci., 60, 1069 (1997), Eur. J. Pharmacol., 357, 235 (1998)].Combined utilization of these chemically synthesized muscarinic toxins together with already commercially available MT1 and MT7, the research concerning biological functions elicited through muscarinic acetylcholine receptors should advance significantly.

Synthetic Product (disulfide bonds between Cys3– Cys24, Cys17– Cys42, Cys46– Cys57 and Cys58– Cys63 )

The purity of muscarinic toxin-3 is guaranteed to be higher than 99% by HPLC